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ezh2 incubator unc 1999  (Tocris)


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    Structured Review

    Tocris ezh2 incubator unc 1999
    ( A ) H3K27me3 ChIP-qPCR in ESCs (Left) and EpiLCs (Middle) in WT and the ∆ mutant. There is no significant effect on polycomb dynamics in ESCs. In EpiLCs, the ∆ mutant retains residual H3K27me3 relative to WT. H3K27me3 ChIP-qPCR in WT and ∆ ESCs and EpiLCs at control loci are displayed in right panel. Data shown as ±s.e.m. from three biological replicates for each genotype. ( B ) Alleles generated by CRISPR/Cas9-mediated mutagenesis of Eed in WT and ∆ contexts. ( C ) Western blot confirming loss of EED protein and H3K27me3 in Eed-/- cell lines. ( D ) Western blot showing no detection of H3K27me3 loss after four days of incubation with PRC2 inhibitor. Negative control exhibited strong H3K27me3 signal. ( E ) RT-qPCR of Zdbf2 in absence of CTCF partition with <t>EZH2</t> inhibitor. In ∆CTCF_PS mutants, there is a further increase of Zdbf2 de-repression when H3K27me3 is depleted. Data shown as ±s.e.m. from three biological replicates for each genotype. Statistical analyses were performed by two-tailed unpaired t-test: *p≤0.05, **≤0.01, ***p≤0.001.
    Ezh2 Incubator Unc 1999, supplied by Tocris, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ezh2 incubator unc 1999/product/Tocris
    Average 91 stars, based on 11 article reviews
    ezh2 incubator unc 1999 - by Bioz Stars, 2026-05
    91/100 stars

    Images

    1) Product Images from "Dynamic enhancer partitioning instructs activation of a growth-related gene during exit from naïve pluripotency"

    Article Title: Dynamic enhancer partitioning instructs activation of a growth-related gene during exit from naïve pluripotency

    Journal: eLife

    doi: 10.7554/eLife.44057

    ( A ) H3K27me3 ChIP-qPCR in ESCs (Left) and EpiLCs (Middle) in WT and the ∆ mutant. There is no significant effect on polycomb dynamics in ESCs. In EpiLCs, the ∆ mutant retains residual H3K27me3 relative to WT. H3K27me3 ChIP-qPCR in WT and ∆ ESCs and EpiLCs at control loci are displayed in right panel. Data shown as ±s.e.m. from three biological replicates for each genotype. ( B ) Alleles generated by CRISPR/Cas9-mediated mutagenesis of Eed in WT and ∆ contexts. ( C ) Western blot confirming loss of EED protein and H3K27me3 in Eed-/- cell lines. ( D ) Western blot showing no detection of H3K27me3 loss after four days of incubation with PRC2 inhibitor. Negative control exhibited strong H3K27me3 signal. ( E ) RT-qPCR of Zdbf2 in absence of CTCF partition with EZH2 inhibitor. In ∆CTCF_PS mutants, there is a further increase of Zdbf2 de-repression when H3K27me3 is depleted. Data shown as ±s.e.m. from three biological replicates for each genotype. Statistical analyses were performed by two-tailed unpaired t-test: *p≤0.05, **≤0.01, ***p≤0.001.
    Figure Legend Snippet: ( A ) H3K27me3 ChIP-qPCR in ESCs (Left) and EpiLCs (Middle) in WT and the ∆ mutant. There is no significant effect on polycomb dynamics in ESCs. In EpiLCs, the ∆ mutant retains residual H3K27me3 relative to WT. H3K27me3 ChIP-qPCR in WT and ∆ ESCs and EpiLCs at control loci are displayed in right panel. Data shown as ±s.e.m. from three biological replicates for each genotype. ( B ) Alleles generated by CRISPR/Cas9-mediated mutagenesis of Eed in WT and ∆ contexts. ( C ) Western blot confirming loss of EED protein and H3K27me3 in Eed-/- cell lines. ( D ) Western blot showing no detection of H3K27me3 loss after four days of incubation with PRC2 inhibitor. Negative control exhibited strong H3K27me3 signal. ( E ) RT-qPCR of Zdbf2 in absence of CTCF partition with EZH2 inhibitor. In ∆CTCF_PS mutants, there is a further increase of Zdbf2 de-repression when H3K27me3 is depleted. Data shown as ±s.e.m. from three biological replicates for each genotype. Statistical analyses were performed by two-tailed unpaired t-test: *p≤0.05, **≤0.01, ***p≤0.001.

    Techniques Used: ChIP-qPCR, Mutagenesis, Control, Generated, CRISPR, Western Blot, Incubation, Negative Control, Quantitative RT-PCR, Two Tailed Test


    Figure Legend Snippet:

    Techniques Used: CRISPR, Generated, Mutagenesis, Negative Control, Recombinant, Software



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    ezh2 incubator unc 1999  (Tocris)
    91
    Tocris ezh2 incubator unc 1999
    ( A ) H3K27me3 ChIP-qPCR in ESCs (Left) and EpiLCs (Middle) in WT and the ∆ mutant. There is no significant effect on polycomb dynamics in ESCs. In EpiLCs, the ∆ mutant retains residual H3K27me3 relative to WT. H3K27me3 ChIP-qPCR in WT and ∆ ESCs and EpiLCs at control loci are displayed in right panel. Data shown as ±s.e.m. from three biological replicates for each genotype. ( B ) Alleles generated by CRISPR/Cas9-mediated mutagenesis of Eed in WT and ∆ contexts. ( C ) Western blot confirming loss of EED protein and H3K27me3 in Eed-/- cell lines. ( D ) Western blot showing no detection of H3K27me3 loss after four days of incubation with PRC2 inhibitor. Negative control exhibited strong H3K27me3 signal. ( E ) RT-qPCR of Zdbf2 in absence of CTCF partition with <t>EZH2</t> inhibitor. In ∆CTCF_PS mutants, there is a further increase of Zdbf2 de-repression when H3K27me3 is depleted. Data shown as ±s.e.m. from three biological replicates for each genotype. Statistical analyses were performed by two-tailed unpaired t-test: *p≤0.05, **≤0.01, ***p≤0.001.
    Ezh2 Incubator Unc 1999, supplied by Tocris, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ezh2 incubator unc 1999/product/Tocris
    Average 91 stars, based on 1 article reviews
    ezh2 incubator unc 1999 - by Bioz Stars, 2026-05
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) H3K27me3 ChIP-qPCR in ESCs (Left) and EpiLCs (Middle) in WT and the ∆ mutant. There is no significant effect on polycomb dynamics in ESCs. In EpiLCs, the ∆ mutant retains residual H3K27me3 relative to WT. H3K27me3 ChIP-qPCR in WT and ∆ ESCs and EpiLCs at control loci are displayed in right panel. Data shown as ±s.e.m. from three biological replicates for each genotype. ( B ) Alleles generated by CRISPR/Cas9-mediated mutagenesis of Eed in WT and ∆ contexts. ( C ) Western blot confirming loss of EED protein and H3K27me3 in Eed-/- cell lines. ( D ) Western blot showing no detection of H3K27me3 loss after four days of incubation with PRC2 inhibitor. Negative control exhibited strong H3K27me3 signal. ( E ) RT-qPCR of Zdbf2 in absence of CTCF partition with EZH2 inhibitor. In ∆CTCF_PS mutants, there is a further increase of Zdbf2 de-repression when H3K27me3 is depleted. Data shown as ±s.e.m. from three biological replicates for each genotype. Statistical analyses were performed by two-tailed unpaired t-test: *p≤0.05, **≤0.01, ***p≤0.001.

    Journal: eLife

    Article Title: Dynamic enhancer partitioning instructs activation of a growth-related gene during exit from naïve pluripotency

    doi: 10.7554/eLife.44057

    Figure Lengend Snippet: ( A ) H3K27me3 ChIP-qPCR in ESCs (Left) and EpiLCs (Middle) in WT and the ∆ mutant. There is no significant effect on polycomb dynamics in ESCs. In EpiLCs, the ∆ mutant retains residual H3K27me3 relative to WT. H3K27me3 ChIP-qPCR in WT and ∆ ESCs and EpiLCs at control loci are displayed in right panel. Data shown as ±s.e.m. from three biological replicates for each genotype. ( B ) Alleles generated by CRISPR/Cas9-mediated mutagenesis of Eed in WT and ∆ contexts. ( C ) Western blot confirming loss of EED protein and H3K27me3 in Eed-/- cell lines. ( D ) Western blot showing no detection of H3K27me3 loss after four days of incubation with PRC2 inhibitor. Negative control exhibited strong H3K27me3 signal. ( E ) RT-qPCR of Zdbf2 in absence of CTCF partition with EZH2 inhibitor. In ∆CTCF_PS mutants, there is a further increase of Zdbf2 de-repression when H3K27me3 is depleted. Data shown as ±s.e.m. from three biological replicates for each genotype. Statistical analyses were performed by two-tailed unpaired t-test: *p≤0.05, **≤0.01, ***p≤0.001.

    Article Snippet: For EZH2 inhibition experiments, the EZH2 incubator UNC 1999 (or its negative control UNC 2400, Tocris Bioscience) was added to media at a 1 μM final concentration for four days.

    Techniques: ChIP-qPCR, Mutagenesis, Control, Generated, CRISPR, Western Blot, Incubation, Negative Control, Quantitative RT-PCR, Two Tailed Test

    Journal: eLife

    Article Title: Dynamic enhancer partitioning instructs activation of a growth-related gene during exit from naïve pluripotency

    doi: 10.7554/eLife.44057

    Figure Lengend Snippet:

    Article Snippet: For EZH2 inhibition experiments, the EZH2 incubator UNC 1999 (or its negative control UNC 2400, Tocris Bioscience) was added to media at a 1 μM final concentration for four days.

    Techniques: CRISPR, Generated, Mutagenesis, Negative Control, Recombinant, Software